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A recent finding shows that soluble protein cargo lipoprotein lipase can also be sorted into sphingomyelin-rich secretory vesicles through binding to an integral membrane protein Syndecan-1, which depends on its TMD length to target sphingomyelin-rich domains (Sundberg et al., 2019)

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The sample also may contain small amounts of residual GST or the GST fusion protein that did not rebind to the column, as well as aggregates or other minor contaminants such as proteolytic fragments or host cell proteins